Human Attractin ELISA Kit PicoKine®

Attractin is a protein that in humans is encoded by the ATRN gene. This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines.

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.