Human ASAH2 ELISA Kit PicoKine®

Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene. Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 154 166 159 163 5.36 3.3% Sample 2 645 652 670 701 667 30.02 4.5% Sample 3 1669 1466 1624 1563 1581 64.8 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 154 166 159 163 5.36 3.3% Sample 1 171 154 166 159 163 5.36 3.3% Sample 2 645 652 670 701 667 30.02 4.5% Sample 2 645 652 670 701 667 30.02 4.5% Sample 3 1669 1466 1624 1563 1581 64.8 4.1% Sample 3 1669 1466 1624 1563 1581 64.8 4.1%

ASAH2; HNAC1; Neutral ceramidase; N-CDase; NCDase; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase [Cleaved into: Neutral ceramidase soluble form]

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.