Human Pro-Cathepsin B ELISA Kit PicoKine®

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 225 269 241 216 238 8.08 3.4% Sample 2 1024 1103 985 1240 1088 26.11 2.4% Sample 3 1858 1956 1785 2006 1901 58.94 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 225 269 241 216 238 8.08 3.4% Sample 1 225 269 241 216 238 8.08 3.4% Sample 2 1024 1103 985 1240 1088 26.11 2.4% Sample 2 1024 1103 985 1240 1088 26.11 2.4% Sample 3 1858 1956 1785 2006 1901 58.94 3.1% Sample 3 1858 1956 1785 2006 1901 58.94 3.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 225 1024 1858 247 1103 1963 Standard deviation 13.28 53.25 91.04 17.54 59.56 133.48 CV (%) 5.9% 5.2% 4.9% 7.1% 5.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 225 1024 1858 247 1103 1963 Standard deviation 13.28 53.25 91.04 17.54 59.56 133.48 CV (%) 5.9% 5.2% 4.9% 7.1% 5.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 225 1024 1858 247 1103 1963 Mean (pg/ml) 225 1024 1858 247 1103 1963 Standard deviation 13.28 53.25 91.04 17.54 59.56 133.48 Standard deviation 13.28 53.25 91.04 17.54 59.56 133.48 CV (%) 5.9% 5.2% 4.9% 7.1% 5.4% 6.8% CV (%) 5.9% 5.2% 4.9% 7.1% 5.4% 6.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.076 0.12 0.255 0.485 1.02 1.455 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.076 0.12 0.255 0.485 1.02 1.455 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.076 0.12 0.255 0.485 1.02 1.455 2.001 O.D. 0.014 0.076 0.12 0.255 0.485 1.02 1.455 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.