Mouse Cathepsin E ELISA Kit PicoKine®

Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 191 188 163 180 12.06 6.7% Sample 2 785 696 814 793 772 37.83 4.9% Sample 3 2784 2863 2924 2652 2806 154.32 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 191 188 163 180 12.06 6.7% Sample 1 178 191 188 163 180 12.06 6.7% Sample 2 785 696 814 793 772 37.83 4.9% Sample 2 785 696 814 793 772 37.83 4.9% Sample 3 2784 2863 2924 2652 2806 154.32 5.5% Sample 3 2784 2863 2924 2652 2806 154.32 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 785 2784 191 805 2845 Standard deviation 7.30 34.54 141.98 10.51 31.40 173.55 CV (%) 4.1% 4.4% 5.1% 5.5% 3.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 785 2784 191 805 2845 Standard deviation 7.30 34.54 141.98 10.51 31.40 173.55 CV (%) 4.1% 4.4% 5.1% 5.5% 3.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 178 785 2784 191 805 2845 Mean (pg/ml) 178 785 2784 191 805 2845 Standard deviation 7.30 34.54 141.98 10.51 31.40 173.55 Standard deviation 7.30 34.54 141.98 10.51 31.40 173.55 CV (%) 4.1% 4.4% 5.1% 5.5% 3.9% 6.1% CV (%) 4.1% 4.4% 5.1% 5.5% 3.9% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.