Human IFNA2 ELISA Kit PicoKine®

Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 5 4 6 5 5 0.11 2.2% Sample 2 19 22 20 15 19 0.51 2.7% Sample 3 69 71 75 80 74 2.58 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 5 4 6 5 5 0.11 2.2% Sample 1 5 4 6 5 5 0.11 2.2% Sample 2 19 22 20 15 19 0.51 2.7% Sample 2 19 22 20 15 19 0.51 2.7% Sample 3 69 71 75 80 74 2.58 3.5% Sample 3 69 71 75 80 74 2.58 3.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 5 19 69 4 21 72 Standard deviation 0.21 0.82 2.69 0.20 1.24 4.10 CV (%) 4.2% 4.3% 3.9% 5.1% 5.9% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 5 19 69 4 21 72 Standard deviation 0.21 0.82 2.69 0.20 1.24 4.10 CV (%) 4.2% 4.3% 3.9% 5.1% 5.9% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 5 19 69 4 21 72 Mean (pg/ml) 5 19 69 4 21 72 Standard deviation 0.21 0.82 2.69 0.20 1.24 4.10 Standard deviation 0.21 0.82 2.69 0.20 1.24 4.10 CV (%) 4.2% 4.3% 3.9% 5.1% 5.9% 5.7% CV (%) 4.2% 4.3% 3.9% 5.1% 5.9% 5.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (pg/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955 O.D. 0.014 0.039 0.076 0.12 0.255 0.485 1.02 1.955

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.