Mouse NGFR/TNFRSF16 ELISA Kit PicoKine®

Nerve growth factor receptor (NGFR) is also referred to as p75 (NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75 (NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75 (NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75 (NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75 (NTR) is also found to activate the SM pathway.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 51 49 53 1.76 3.3% Sample 2 299 281 277 308 291 10.78 3.7% Sample 3 841 873 791 834 835 34.22 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 51 49 53 1.76 3.3% Sample 1 55 58 51 49 53 1.76 3.3% Sample 2 299 281 277 308 291 10.78 3.7% Sample 2 299 281 277 308 291 10.78 3.7% Sample 3 841 873 791 834 835 34.22 4.1% Sample 3 841 873 791 834 835 34.22 4.1%

Ngfr; Tnfrsf16; Tumor necrosis factor receptor superfamily member 16; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; CD antigen CD271

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 299 841 61 263 907 Standard deviation 4.07 20.63 47.10 4.33 20.78 76.19 CV (%) 7.4% 6.9% 5.6% 7.1% 7.9% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 299 841 61 263 907 Standard deviation 4.07 20.63 47.10 4.33 20.78 76.19 CV (%) 7.4% 6.9% 5.6% 7.1% 7.9% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 299 841 61 263 907 Mean (pg/ml) 55 299 841 61 263 907 Standard deviation 4.07 20.63 47.10 4.33 20.78 76.19 Standard deviation 4.07 20.63 47.10 4.33 20.78 76.19 CV (%) 7.4% 6.9% 5.6% 7.1% 7.9% 8.4% CV (%) 7.4% 6.9% 5.6% 7.1% 7.9% 8.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.068 0.11 0.183 0.317 0.588 1.068 1.829 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.068 0.11 0.183 0.317 0.588 1.068 1.829 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.068 0.11 0.183 0.317 0.588 1.068 1.829 O.D. 0.022 0.068 0.11 0.183 0.317 0.588 1.068 1.829

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.