Mouse Ncan/Neurocan ELISA Kit PicoKine®

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 100 106 98 105 8.25 7.9% Sample 2 127 126 236 140 157 8.82 5.6% Sample 3 1709 1996 1988 1057 1688 76.11 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 100 106 98 105 8.25 7.9% Sample 1 117 100 106 98 105 8.25 7.9% Sample 2 127 126 236 140 157 8.82 5.6% Sample 2 127 126 236 140 157 8.82 5.6% Sample 3 1709 1996 1988 1057 1688 76.11 4.5% Sample 3 1709 1996 1988 1057 1688 76.11 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.