Mouse Ncan/Neurocan ELISA Kit PicoKine®

Mouse Ncan/Neurocan ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ncan in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 100 106 98 105 8.25 7.9% Sample 2 127 126 236 140 157 8.82 5.6% Sample 3 1709 1996 1988 1057 1688 76.11 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 117 100 106 98 105 8.25 7.9% Sample 1 117 100 106 98 105 8.25 7.9% Sample 2 127 126 236 140 157 8.82 5.6% Sample 2 127 126 236 140 157 8.82 5.6% Sample 3 1709 1996 1988 1057 1688 76.11 4.5% Sample 3 1709 1996 1988 1057 1688 76.11 4.5%

Gene Name

Ncan

Gene ID

13004

UniProt

P55066

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: D23-D637

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 117 127 1709 118 126 1060 Mean (pg/ml) 117 127 1709 118 126 1060 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 Standard deviation 7.53 9.06 124.59 8.67 3.06 60.1 CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7% CV (%) 6.4% 7.1% 7.3% 7.4% 2.4% 5.7%

Sensitivity

<30 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Ncan

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089 O.D. 0.068 0.157 0.224 0.366 0.592 0.922 1.524 2.089

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.