Human NCAN/Neurocan ELISA Kit PicoKine®

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 88 85 87 4.52 5.2% Sample 2 221 258 211 247 234 18.02 7.7% Sample 3 972 965 933 954 956 80.30 8.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 88 85 87 4.52 5.2% Sample 1 83 91 88 85 87 4.52 5.2% Sample 2 221 258 211 247 234 18.02 7.7% Sample 2 221 258 211 247 234 18.02 7.7% Sample 3 972 965 933 954 956 80.30 8.4% Sample 3 972 965 933 954 956 80.30 8.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.