Human LILRA5/CD85f ELISA Kit PicoKine®

The protein encoded by this gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. LIR family members are known to have activating and inibitory functions in leukocytes. Crosslink of this receptor protein on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses. This gene is one of the leukocyte receptor genes that form a gene cluster on the chromosomal region 19q13.4. Four alternatively spliced transcript variants encoding distinct isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 163 175 99 168 151 3.45 2.3% Sample 2 750 413 597 595 589 30.03 5.1% Sample 3 2098 1503 2488 2724 2203 172.73 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 163 175 99 168 151 3.45 2.3% Sample 1 163 175 99 168 151 3.45 2.3% Sample 2 750 413 597 595 589 30.03 5.1% Sample 2 750 413 597 595 589 30.03 5.1% Sample 3 2098 1503 2488 2724 2203 172.73 7.8% Sample 3 2098 1503 2488 2724 2203 172.73 7.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 163 750 2098 125 663 2502 Standard deviation 8.56 37.05 91.47 6.01 16.84 152.12 CV (%) 5.3% 4.9% 4.4% 4.8% 2.5% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 163 750 2098 125 663 2502 Standard deviation 8.56 37.05 91.47 6.01 16.84 152.12 CV (%) 5.3% 4.9% 4.4% 4.8% 2.5% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 163 750 2098 125 663 2502 Mean (pg/ml) 163 750 2098 125 663 2502 Standard deviation 8.56 37.05 91.47 6.01 16.84 152.12 Standard deviation 8.56 37.05 91.47 6.01 16.84 152.12 CV (%) 5.3% 4.9% 4.4% 4.8% 2.5% 6.1% CV (%) 5.3% 4.9% 4.4% 4.8% 2.5% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.049 0.138 0.186 0.273 0.479 0.853 1.426 2.059 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.049 0.138 0.186 0.273 0.479 0.853 1.426 2.059 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.049 0.138 0.186 0.273 0.479 0.853 1.426 2.059 O.D. 0.049 0.138 0.186 0.273 0.479 0.853 1.426 2.059

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.