Human LILRA3/CD85e ELISA Kit PicoKine®

This gene encodes a member of a family of immunoreceptors that are expressed predominantly in monocytes and B cells, and at lower levels in dendritic cells and natural killer cells. The encoded protein lacks the transmembrane region found in other members of this family. It acts as a soluble receptor for class I major histocompatibility complex (MHC) antigens. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and is polymorphic in human populations, with many individuals containing a deletion of this genomic region.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 38 41 45 44 1.95 4.4% Sample 2 284 285 271 266 277 8.85 3.2% Sample 3 914 898 906 923 910 44.60 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 38 41 45 44 1.95 4.4% Sample 1 58 38 41 45 44 1.95 4.4% Sample 2 284 285 271 266 277 8.85 3.2% Sample 2 284 285 271 266 277 8.85 3.2% Sample 3 914 898 906 923 910 44.60 4.9% Sample 3 914 898 906 923 910 44.60 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 284 914 51 322 942 Mean (pg/ml) 53 284 914 51 322 942 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 Standard deviation 2.28 10.79 44.79 2.65 18.03 48.04 CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1% CV (%) 4.3% 3.8% 4.9% 5.2% 5.6% 5.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.079 0.139 0.253 0.471 0.842 1.437 2.527 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.079 0.139 0.253 0.471 0.842 1.437 2.527 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.079 0.139 0.253 0.471 0.842 1.437 2.527 O.D. 0.01 0.079 0.139 0.253 0.471 0.842 1.437 2.527

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.