Rat Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 226 217 208 243 224 10.53 4.7% Sample 2 813 835 829 846 831 49.03 5.9% Sample 3 1749 1876 1816 1721 1791 110.98 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 226 217 208 243 224 10.53 4.7% Sample 1 226 217 208 243 224 10.53 4.7% Sample 2 813 835 829 846 831 49.03 5.9% Sample 2 813 835 829 846 831 49.03 5.9% Sample 3 1749 1876 1816 1721 1791 110.98 6.2% Sample 3 1749 1876 1816 1721 1791 110.98 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 226 813 1749 235 854 1806 Standard deviation 10.62 45.53 101.44 15.04 62.34 140.87 CV (%) 4.7% 5.6% 5.8% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 226 813 1749 235 854 1806 Standard deviation 10.62 45.53 101.44 15.04 62.34 140.87 CV (%) 4.7% 5.6% 5.8% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 226 813 1749 235 854 1806 Mean (pg/ml) 226 813 1749 235 854 1806 Standard deviation 10.62 45.53 101.44 15.04 62.34 140.87 Standard deviation 10.62 45.53 101.44 15.04 62.34 140.87 CV (%) 4.7% 5.6% 5.8% 6.4% 7.3% 7.8% CV (%) 4.7% 5.6% 5.8% 6.4% 7.3% 7.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.169 0.263 0.476 0.878 1.725 2.429 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.169 0.263 0.476 0.878 1.725 2.429 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.047 0.112 0.169 0.263 0.476 0.878 1.725 2.429 O.D. 0.047 0.112 0.169 0.263 0.476 0.878 1.725 2.429

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.