Human Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

Human Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (96 Tests) . Quantitate Human BMPER in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 198 182 204 212 199 10.15 5.1% Sample 2 763 782 792 790 782 48.48 6.2% Sample 3 1376 1294 1453 1380 1376 67.42 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 198 182 204 212 199 10.15 5.1% Sample 1 198 182 204 212 199 10.15 5.1% Sample 2 763 782 792 790 782 48.48 6.2% Sample 2 763 782 792 790 782 48.48 6.2% Sample 3 1376 1294 1453 1380 1376 67.42 4.9% Sample 3 1376 1294 1453 1380 1376 67.42 4.9%

Gene Name

BMPER

Gene ID

168667

UniProt

Q8N8U9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V34-R685

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 198 763 1376 205 781 1424 Standard deviation 11.09 46.54 88.06 14.76 58.58 119.62 CV (%) 5.6% 6.1% 6.4% 7.2% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 198 763 1376 205 781 1424 Standard deviation 11.09 46.54 88.06 14.76 58.58 119.62 CV (%) 5.6% 6.1% 6.4% 7.2% 7.5% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 198 763 1376 205 781 1424 Mean (pg/ml) 198 763 1376 205 781 1424 Standard deviation 11.09 46.54 88.06 14.76 58.58 119.62 Standard deviation 11.09 46.54 88.06 14.76 58.58 119.62 CV (%) 5.6% 6.1% 6.4% 7.2% 7.5% 8.4% CV (%) 5.6% 6.1% 6.4% 7.2% 7.5% 8.4%

Sensitivity

<50 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human BMPER

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.085 0.162 0.294 0.546 0.952 1.573 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.085 0.162 0.294 0.546 0.952 1.573 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.017 0.085 0.162 0.294 0.546 0.952 1.573 2.089 O.D. 0.017 0.085 0.162 0.294 0.546 0.952 1.573 2.089

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.