Human LAIR1 ELISA Kit PicoKine®

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 208 212 206 209 12.96 6.2% Sample 2 976 962 985 967 973 65.19 6.7% Sample 3 1958 1930 1942 1964 1948.5 140.29 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 208 212 206 209 12.96 6.2% Sample 1 210 208 212 206 209 12.96 6.2% Sample 2 976 962 985 967 973 65.19 6.7% Sample 2 976 962 985 967 973 65.19 6.7% Sample 3 1958 1930 1942 1964 1948.5 140.29 7.2% Sample 3 1958 1930 1942 1964 1948.5 140.29 7.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 976 1958 216 989 1970 Standard deviation 11.34 67.34 140.98 15.55 84.07 181.24 CV (%) 5.4% 6.9% 7.2% 7.2% 8.5% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 976 1958 216 989 1970 Standard deviation 11.34 67.34 140.98 15.55 84.07 181.24 CV (%) 5.4% 6.9% 7.2% 7.2% 8.5% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 210 976 1958 216 989 1970 Mean (pg/ml) 210 976 1958 216 989 1970 Standard deviation 11.34 67.34 140.98 15.55 84.07 181.24 Standard deviation 11.34 67.34 140.98 15.55 84.07 181.24 CV (%) 5.4% 6.9% 7.2% 7.2% 8.5% 9.2% CV (%) 5.4% 6.9% 7.2% 7.2% 8.5% 9.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.134 0.209 0.349 0.604 0.956 1.537 2.064 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.134 0.209 0.349 0.604 0.956 1.537 2.064 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.134 0.209 0.349 0.604 0.956 1.537 2.064 O.D. 0.055 0.134 0.209 0.349 0.604 0.956 1.537 2.064

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.