Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. It is mapped to 5 E5; 5 50.55 cM. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 467 427 448 472 453 17.72 3.9% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 3 7832 7176 7216 8274 7624 456.32 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 467 427 448 472 453 17.72 3.9% Sample 1 467 427 448 472 453 17.72 3.9% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 3 7832 7176 7216 8274 7624 456.32 5.9% Sample 3 7832 7176 7216 8274 7624 456.32 5.9%

Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.