Human LILRB2 ELISA Kit PicoKine®

Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 384 401 375 392 388 9.61 2.4% Sample 2 2313 1997 2156 1982 2112 134.59 6.3% Sample 3 8445 7435 7826 7234 7735 461.89 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 384 401 375 392 388 9.61 2.4% Sample 1 384 401 375 392 388 9.61 2.4% Sample 2 2313 1997 2156 1982 2112 134.59 6.3% Sample 2 2313 1997 2156 1982 2112 134.59 6.3% Sample 3 8445 7435 7826 7234 7735 461.89 5.9% Sample 3 8445 7435 7826 7234 7735 461.89 5.9%

Leukocyte immunoglobulin-like receptor subfamily B member 2; LIR-2; Leukocyte immunoglobulin-like receptor 2; CD85 antigen-like family member D; Immunoglobulin-like transcript 4; ILT-4; Monocyte/macrophage immunoglobulin-like receptor 10; MIR-10; CD85d; LILRB2; ILT4; LIR2; MIR10

Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells. Expressed in myeloid-derived suppressor cells during pregnancy. Detected at low levels in natural killer (NK) cells. Expressed in B cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 384 2313 8445 492 2436 9458 Standard deviation 17.28 131.84 388.47 25.09 143.72 699.89 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 384 2313 8445 492 2436 9458 Standard deviation 17.28 131.84 388.47 25.09 143.72 699.89 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 384 2313 8445 492 2436 9458 Mean (pg/ml) 384 2313 8445 492 2436 9458 Standard deviation 17.28 131.84 388.47 25.09 143.72 699.89 Standard deviation 17.28 131.84 388.47 25.09 143.72 699.89 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4%

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078 O.D. 0.032 0.118 0.184 0.333 0.593 1.041 1.706 2.078

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.