Human LILRB1 ELISA Kit PicoKine®

Leukocyte immunoglobulin-like receptors (LILRs), also called CD85s, ILTs, or LIRs, are important mediators of immune activation and tolerance that contain tandem immunoglobulin (Ig) -like folds. LILRB1 is encoded within the leukocyte receptor complex on 19q13.4, previously implicated to be a susceptibility region to systemic lupus erythematosus (SLE) .LILRB1 binds to a wide range of major histocompatibility complex class I molecules (MHCIs) and transduces negative signals that can, for example, prevent killing of MHCI-expressing cells.LILRB1 transcription is correlated with surface protein expression on NK cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 66 64 100 85 3.48 4.1% Sample 2 162 250 141 229 196 8.31 4.3% Sample 3 1569 1200 1604 1340 1428 54.13 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 66 64 100 85 3.48 4.1% Sample 1 110 66 64 100 85 3.48 4.1% Sample 2 162 250 141 229 196 8.31 4.3% Sample 2 162 250 141 229 196 8.31 4.3% Sample 3 1569 1200 1604 1340 1428 54.13 3.8% Sample 3 1569 1200 1604 1340 1428 54.13 3.8%

Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; CD85j; LILRB1; ILT2; LIR1; MIR7

Expressed in B cells, monocytes and various dendritic cell (DC) subsets including myeloid, plasmacytoid and tolerogenic DCs (at protein level) . Expressed in decidual macrophages (at protein level) . Expressed in decidual NK cells (at protein level) .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 162 1569 116 182 1572 Standard deviation 8.34 9.95 44.56 8.97 12.94 76.08 CV (%) 7.6% 6.1% 2.8% 7.7% 7.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 162 1569 116 182 1572 Standard deviation 8.34 9.95 44.56 8.97 12.94 76.08 CV (%) 7.6% 6.1% 2.8% 7.7% 7.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 162 1569 116 182 1572 Mean (pg/ml) 110 162 1569 116 182 1572 Standard deviation 8.34 9.95 44.56 8.97 12.94 76.08 Standard deviation 8.34 9.95 44.56 8.97 12.94 76.08 CV (%) 7.6% 6.1% 2.8% 7.7% 7.1% 4.8% CV (%) 7.6% 6.1% 2.8% 7.7% 7.1% 4.8%

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide. Upon interaction with peptide-bound HLA-G-B2M complex, triggers secretion of growth-promoting factors by decidual NK cells. Reprograms B cells toward an immune suppressive phenotype.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.092 0.164 0.285 0.577 0.964 1.523 2.138 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.092 0.164 0.285 0.577 0.964 1.523 2.138 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.092 0.164 0.285 0.577 0.964 1.523 2.138 O.D. 0.041 0.092 0.164 0.285 0.577 0.964 1.523 2.138

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.