Mouse CD44 ELISA Kit PicoKine®

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 723 672 750 655 700 38.2 5.4% Sample 2 1936 1948 1970 2114 1992 71.48 3.5% Sample 3 8300 7750 8088 8319 8114 229.01 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 723 672 750 655 700 38.2 5.4% Sample 1 723 672 750 655 700 38.2 5.4% Sample 2 1936 1948 1970 2114 1992 71.48 3.5% Sample 2 1936 1948 1970 2114 1992 71.48 3.5% Sample 3 8300 7750 8088 8319 8114 229.01 2.8% Sample 3 8300 7750 8088 8319 8114 229.01 2.8%

CD44 antigen; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Lymphocyte antigen 24; Ly-24; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD44; Cd44; Ly-24

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Cancer, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Mesenchymal Stem Cells, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage, Tumor Biomarkers, Tumor Immunology

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 723 1936 8300 711 1975 7916 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 CV (%) 6.7% 7.4% 6.8% 8.6% 9.1% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 723 1936 8300 711 1975 7916 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 CV (%) 6.7% 7.4% 6.8% 8.6% 9.1% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 723 1936 8300 711 1975 7916 Mean (pg/ml) 723 1936 8300 711 1975 7916 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 Standard deviation 48.44 143.26 564.4 61.14 179.72 585.78 CV (%) 6.7% 7.4% 6.8% 8.6% 9.1% 7.4% CV (%) 6.7% 7.4% 6.8% 8.6% 9.1% 7.4%

Receptor for hyaluronic acid (HA) . Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis (By similarity) . Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.193 0.228 0.308 0.508 0.854 1.509 2.357 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.193 0.228 0.308 0.508 0.854 1.509 2.357 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.193 0.228 0.308 0.508 0.854 1.509 2.357 O.D. 0.036 0.193 0.228 0.308 0.508 0.854 1.509 2.357

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.