Human CANT1 ELISA Kit PicoKine®

CANT1?is a?gene?that encodes soluble calcium-activated?nucleotidase?1, an?enzyme, in humans. It is mapped to 17q25.3. This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Sample 3 5570 5244 5973 5762 5637 268.07 4.7%

Soluble calcium-activated nucleotidase 1; SCAN-1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; CANT1; SHAPY

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8%

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15248776, PubMed:15006348, PubMed:16835225) . Involved in proteoglycan synthesis (PubMed:22539336) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.