Human ANTXR2 ELISA Kit PicoKine®

Anthrax toxin receptor 2?is a?protein?that in humans is encoded by the?ANTXR2?gene. It is mapped to 4q21.21. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3%

Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.