Human ANTXR2 ELISA Kit PicoKine®

Human ANTXR2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Anthrax toxin receptor 2?is a?protein?that in humans is encoded by the?ANTXR2?gene. It is mapped to 4q21.21. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3%

Synonyms

Anthrax toxin receptor 2; Capillary morphogenesis gene 2 protein; CMG-2; ANTXR2; CMG2

Gene Name

ANTXR2

Gene ID

118429

UniProt

P58335

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q34-G318

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated monoclonal antibody from goat

Tissue Specificity

Expressed in prostate, thymus, ovary, testis, pancreas, colon, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, skeletal muscle, small intestine and spleen.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

Sensitivity

<50 pg/mL

Function

Necessary for cellular interactions with laminin and the extracellular matrix.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ANTXR2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

ANTXR cell adhesion molecule 2

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Secreted

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051 O.D. 0.046 0.15 0.22 0.449 0.788 1.217 1.809 2.051

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.