Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Plasma kallikrein?is a?protein?that in humans is encoded by the?KLKB1?gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 275 235 272 258 16.19 6.2% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 3 4296 4210 3832 4617 4238 279.56 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 275 235 272 258 16.19 6.2% Sample 1 252 275 235 272 258 16.19 6.2% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 3 4296 4210 3832 4617 4238 279.56 6.5% Sample 3 4296 4210 3832 4617 4238 279.56 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1236 4806 295 1245 4921 Mean (pg/ml) 247 1236 4806 295 1245 4921 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 Standard deviation 11.12 70.45 221.08 20.95 85.91 314.94 CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4% CV (%) 4.5% 5.7% 4.6% 7.1% 6.9% 6.4%

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.13 0.21 0.373 0.686 1.174 1.743 2.333 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.13 0.21 0.373 0.686 1.174 1.743 2.333 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.13 0.21 0.373 0.686 1.174 1.743 2.333 O.D. 0.055 0.13 0.21 0.373 0.686 1.174 1.743 2.333

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.