Mouse PCOLCE ELISA Kit PicoKine®

Mouse PCOLCE ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse PCOLCE in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. It is mapped to 7q22.1. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Sample 3 5631 5520 5145 4973 5317 268.19 5%

Synonyms

Procollagen C-endopeptidase enhancer 1; P14; Procollagen COOH-terminal proteinase enhancer 1; PCPE-1; Procollagen C-proteinase enhancer 1; Type 1 procollagen C-proteinase enhancer protein; Type I procollagen COOH-terminal proteinase enhancer; Pcolce; Pcpe1

Gene Name

PCOLCE

Gene ID

18542

UniProt

Q61398

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-A468

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated monoclonal antibody from goat

Tissue Specificity

Expressed in interstitial connective tissues like tendons, calvaria, skin and at a lower level in heart and skeletal muscle.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6%

Sensitivity

<50 pg/mL

Function

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse PCOLCE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Procollagen C-endopeptidase enhancer protein

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.085 0.142 0.266 0.494 0.865 1.493 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.085 0.142 0.266 0.494 0.865 1.493 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.085 0.142 0.266 0.494 0.865 1.493 2.2 O.D. 0.019 0.085 0.142 0.266 0.494 0.865 1.493 2.2

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.