Human QPCT ELISA Kit PicoKine®

Glutaminyl-peptide cyclotransferase is an enzyme that in humans is encoded by the QPCT gene. It is mapped to 2p22.2. This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 266 300 267 274 14.73 5.3% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 3 4789 4996 5212 5707 5176 341.11 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 266 300 267 274 14.73 5.3% Sample 1 265 266 300 267 274 14.73 5.3% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 3 4789 4996 5212 5707 5176 341.11 6.5% Sample 3 4789 4996 5212 5707 5176 341.11 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8%

Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.168 0.268 0.472 0.781 1.242 1.911 2.306 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.168 0.268 0.472 0.781 1.242 1.911 2.306 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.168 0.268 0.472 0.781 1.242 1.911 2.306 O.D. 0.023 0.168 0.268 0.472 0.781 1.242 1.911 2.306

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.