Human APLP1 ELISA Kit PicoKine®

This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 209 251 225 234 18.15 7.7% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 3 5201 4542 5134 5110 4996 264.65 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 209 251 225 234 18.15 7.7% Sample 1 252 209 251 225 234 18.15 7.7% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 3 5201 4542 5134 5110 4996 264.65 5.2% Sample 3 5201 4542 5134 5110 4996 264.65 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 268 970 3393 309 1028 4064 Mean (pg/ml) 268 970 3393 309 1028 4064 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 Standard deviation 10.99 41.71 156.08 17.92 70.93 292.61 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity) . Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.