Rat Flt-3 ligand ELISA Kit PicoKine®

Rat Flt-3 ligand ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Flt3lg in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1) . In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 33 34 35 1.58 4.5% Sample 2 160 162 184 182 172 11.04 6.4% Sample 3 699 680 621 637 659 31.49 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 33 34 35 1.58 4.5% Sample 1 36 37 33 34 35 1.58 4.5% Sample 2 160 162 184 182 172 11.04 6.4% Sample 2 160 162 184 182 172 11.04 6.4% Sample 3 699 680 621 637 659 31.49 4.7% Sample 3 699 680 621 637 659 31.49 4.7%

Synonyms

Fms-related tyrosine kinase 3 ligand; Flt3lg

Gene Name

Flt3lg

Gene ID

103691134

UniProt

A0A1W2Q6J3

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G27-R188

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat Flt-3 ligand

Gene Name Synonym

Fms-related tyrosine kinase 3 ligand

Subcellular Location

Extracellular space. Intrinsic component of external side of plasma membrane. Integral component of membrane.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.