Human Erythropoietin/EPO ELISA Kit PicoKine®

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

Lots Lot 1 (mIU/ml) Lot 2 (mIU/ml) Lot 3 (mIU/ml) Lot 4 (mIU/ml) Mean (mIU/ml) Standard Deviation CV (%) Sample 1 4 4 4 4 4 0.00 0.0% Sample 2 23 23 23 21 22 0.86 3.9% Sample 3 68 73 76 65 70 4.27 6.1% Lots Lot 1 (mIU/ml) Lot 2 (mIU/ml) Lot 3 (mIU/ml) Lot 4 (mIU/ml) Mean (mIU/ml) Standard Deviation CV (%) Lots Lot 1 (mIU/ml) Lot 2 (mIU/ml) Lot 3 (mIU/ml) Lot 4 (mIU/ml) Mean (mIU/ml) Standard Deviation CV (%) Sample 1 4 4 4 4 4 0.00 0.0% Sample 1 4 4 4 4 4 0.00 0.0% Sample 2 23 23 23 21 22 0.86 3.9% Sample 2 23 23 23 21 22 0.86 3.9% Sample 3 68 73 76 65 70 4.27 6.1% Sample 3 68 73 76 65 70 4.27 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (mIU/ml) 4 23 68 5 24 76 Standard deviation 0.17 0.99 2.79 0.33 1.63 5.47 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (mIU/ml) 4 23 68 5 24 76 Standard deviation 0.17 0.99 2.79 0.33 1.63 5.47 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (mIU/ml) 4 23 68 5 24 76 Mean (mIU/ml) 4 23 68 5 24 76 Standard deviation 0.17 0.99 2.79 0.33 1.63 5.47 Standard deviation 0.17 0.99 2.79 0.33 1.63 5.47 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.2% CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.2%

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (mIU/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.082 0.14 0.298 0.553 0.967 1.427 2.142 Concentration (mIU/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.082 0.14 0.298 0.553 0.967 1.427 2.142 Concentration (mIU/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (mIU/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.003 0.082 0.14 0.298 0.553 0.967 1.427 2.142 O.D. 0.003 0.082 0.14 0.298 0.553 0.967 1.427 2.142

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.