Rat Cadherin-6/CDH6 ELISA Kit PicoKine®

Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 2q16. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 215 198 199 201 7.79 3.8% Sample 2 980 991 887 890 937 48.66 5.1% Sample 3 3616 3729 3935 3632 3728 127.09 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 215 198 199 201 7.79 3.8% Sample 1 195 215 198 199 201 7.79 3.8% Sample 2 980 991 887 890 937 48.66 5.1% Sample 2 980 991 887 890 937 48.66 5.1% Sample 3 3616 3729 3935 3632 3728 127.09 3.4% Sample 3 3616 3729 3935 3632 3728 127.09 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.