Human Cadherin-6/CDH6 ELISA Kit PicoKine®

Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 5p13.3. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 180 203 210 195 11.86 6.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 3 3740 3185 3099 3077 3275 271.34 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 180 203 210 195 11.86 6.0% Sample 1 188 180 203 210 195 11.86 6.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 3 3740 3185 3099 3077 3275 271.34 8.2% Sample 3 3740 3185 3099 3077 3275 271.34 8.2%

Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.