Rat R-Cadherin-4 Cdh4 ELISA Kit PicoKine®

Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 3q43. This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. The encoded protein is involved in retinal angiogenesis during development where it plays a crucial role in the endothelial-astrocyte interactions. Alternative splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 211 213 197 205 6.68 3.2% Sample 2 941 940 923 856 915 34.80 3.8% Sample 3 4494 5291 4840 4681 4826 294.81 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 211 213 197 205 6.68 3.2% Sample 1 201 211 213 197 205 6.68 3.2% Sample 2 941 940 923 856 915 34.80 3.8% Sample 2 941 940 923 856 915 34.80 3.8% Sample 3 4494 5291 4840 4681 4826 294.81 6.1% Sample 3 4494 5291 4840 4681 4826 294.81 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 941 4494 283 1016 4738 Standard deviation 8.44 40.46 184.25 18.68 73.15 360.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 941 4494 283 1016 4738 Standard deviation 8.44 40.46 184.25 18.68 73.15 360.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 201 941 4494 283 1016 4738 Mean (pg/ml) 201 941 4494 283 1016 4738 Standard deviation 8.44 40.46 184.25 18.68 73.15 360.09 Standard deviation 8.44 40.46 184.25 18.68 73.15 360.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.176 0.236 0.415 0.671 1.097 1.657 2.086 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.176 0.236 0.415 0.671 1.097 1.657 2.086 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.176 0.236 0.415 0.671 1.097 1.657 2.086 O.D. 0.049 0.176 0.236 0.415 0.671 1.097 1.657 2.086

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.