Mouse IL4R ELISA Kit PicoKine®

The interleukin 4 receptor is a type I cytokine receptor. IL4R is its human gene. It is mapped to 7 F3; 7 68.94 cM. This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 248 218 240 257 240 14.44 6.0% Sample 2 842 744 801 849 809 41.76 5.1% Sample 3 4743 5103 4943 4393 4795 265.08 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 248 218 240 257 240 14.44 6.0% Sample 1 248 218 240 257 240 14.44 6.0% Sample 2 842 744 801 849 809 41.76 5.1% Sample 2 842 744 801 849 809 41.76 5.1% Sample 3 4743 5103 4943 4393 4795 265.08 5.5% Sample 3 4743 5103 4943 4393 4795 265.08 5.5%

Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP; Il4r; Il4ra

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 248 842 4743 302 904 4791 Standard deviation 12.65 44.63 265.61 19.93 61.47 340.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 248 842 4743 302 904 4791 Standard deviation 12.65 44.63 265.61 19.93 61.47 340.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 248 842 4743 302 904 4791 Mean (pg/ml) 248 842 4743 302 904 4791 Standard deviation 12.65 44.63 265.61 19.93 61.47 340.16 Standard deviation 12.65 44.63 265.61 19.93 61.47 340.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1%

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.17 0.311 0.6258 1.084 1.772 2.293 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.17 0.311 0.6258 1.084 1.772 2.293 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.17 0.311 0.6258 1.084 1.772 2.293 O.D. 0.006 0.074 0.17 0.311 0.6258 1.084 1.772 2.293

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.