Human IL4R ELISA Kit PicoKine®

The interleukin 4 receptor is a type I cytokine receptor. IL4R is its human gene. It is mapped to 16p12.1. This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 91 79 93 85 6.79 7.9% Sample 2 286 332 287 301 301 18.58 6.1% Sample 3 1170 1113 1219 1099 1150 47.77 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 91 79 93 85 6.79 7.9% Sample 1 78 91 79 93 85 6.79 7.9% Sample 2 286 332 287 301 301 18.58 6.1% Sample 2 286 332 287 301 301 18.58 6.1% Sample 3 1170 1113 1219 1099 1150 47.77 4.1% Sample 3 1170 1113 1219 1099 1150 47.77 4.1%

Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP; IL4R; IL4RA; 582J2.1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 286 1170 81 312 1225 Standard deviation 3.12 12.30 53.82 4.13 17.47 72.28 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 286 1170 81 312 1225 Standard deviation 3.12 12.30 53.82 4.13 17.47 72.28 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 286 1170 81 312 1225 Mean (pg/ml) 78 286 1170 81 312 1225 Standard deviation 3.12 12.30 53.82 4.13 17.47 72.28 Standard deviation 3.12 12.30 53.82 4.13 17.47 72.28 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9%

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.072 0.137 0.264 0.521 1.084 1.892 2.555 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.072 0.137 0.264 0.521 1.084 1.892 2.555 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.072 0.137 0.264 0.521 1.084 1.892 2.555 O.D. 0.007 0.072 0.137 0.264 0.521 1.084 1.892 2.555

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.