What is the catalog number for Human CD99L2 ELISA Kit PicoKine®?

The catalog number for Human CD99L2 ELISA Kit PicoKine® is EK2090.

What sizes are available for Human CD99L2 ELISA Kit PicoKine®?

Human CD99L2 ELISA Kit PicoKine® is available in the following size(s): 96 Well With Removable Strips.

Is Human CD99L2 ELISA Kit PicoKine® in stock?

Human CD99L2 ELISA Kit PicoKine® is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Human CD99L2 ELISA Kit PicoKine®?

The safety data sheet for Human CD99L2 ELISA Kit PicoKine® is available here: https://www.bosterbio.com/msds?sku=EK2090.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

Human CD99L2 ELISA Kit PicoKine®

Human CD99L2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD99L2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to Xq28. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Sample 3 7370 7573 8346 7810 7774 364.72 4.6%

Synonyms

CD99 antigen-like protein 2; MIC2-like protein 1; CD99; CD99L2; MIC2L1; UNQ1964; PRO4486

Gene Name

CD99L2

Gene ID

83692

UniProt

Q8TCZ2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-G185

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in many tissues, with low expression in thymus.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

Sensitivity

<50 pg/mL

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD99L2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

CD99 molecule like 2

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.