Human CD99L2 ELISA Kit PicoKine®

Human CD99L2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD99L2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD99 antigen-like protein 2 is a protein that in humans is encoded by the CD99L2 gene. It is mapped to Xq28. This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Sample 3 7370 7573 8346 7810 7774 364.72 4.6%

Synonyms

CD99 antigen-like protein 2; MIC2-like protein 1; CD99; CD99L2; MIC2L1; UNQ1964; PRO4486

Gene Name

CD99L2

Gene ID

83692

UniProt

Q8TCZ2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D26-G185

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in many tissues, with low expression in thymus.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

Sensitivity

<50 pg/mL

Function

Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD99L2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

CD99 molecule like 2

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Extracellular side. Cell junction.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892 O.D. 0.007 0.045 0.082 0.137 0.244 0.521 1.084 1.892

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.