Mouse CD99 ELISA Kit PicoKine®

CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. It is mapped to 4; 4. The protein has a mass of 32 kD. Unusually for a gene present on the X chromosome, the CD99 gene does not undergo X inactivation, and it was the first such pseudoautosomal gene to be discovered in humans.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 167 180 162 165 9.75 5.9% Sample 2 549 593 548 547 559 19.49 3.4% Sample 3 2842 2852 2882 2685 2815 76.62 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 167 180 162 165 9.75 5.9% Sample 1 153 167 180 162 165 9.75 5.9% Sample 2 549 593 548 547 559 19.49 3.4% Sample 2 549 593 548 547 559 19.49 3.4% Sample 3 2842 2852 2882 2685 2815 76.62 2.7% Sample 3 2842 2852 2882 2685 2815 76.62 2.7%

Widely expressed with high levels in lung, spleen, thymus, liver and spinal chord. Expressed on leukocytes and endothelial cell contacts. Detected in a wide range of T-cells, including CD4-/CD8- and CD4+/CD8+ thymocytes. Expression is much lower in peripheral T-cells than in thymocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 549 2842 214 654 3861 Standard deviation 6.27 23.61 130.73 10.91 38.59 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 549 2842 214 654 3861 Standard deviation 6.27 23.61 130.73 10.91 38.59 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 153 549 2842 214 654 3861 Mean (pg/ml) 153 549 2842 214 654 3861 Standard deviation 6.27 23.61 130.73 10.91 38.59 258.69 Standard deviation 6.27 23.61 130.73 10.91 38.59 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7%

Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.03 0.112 0.169 0.302 0.635 1.067 1.767 2.27 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.03 0.112 0.169 0.302 0.635 1.067 1.767 2.27 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.03 0.112 0.169 0.302 0.635 1.067 1.767 2.27 O.D. 0.03 0.112 0.169 0.302 0.635 1.067 1.767 2.27

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.