Human CRTAC1 ELISA Kit PicoKine®

Cartilage acidic protein 1 is a protein that in humans is encoded by the CRTAC1 gene. It is mapped to 10q24.2. This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 561 555 488 512 529 30.29 5.7% Sample 2 1709 1635 1676 1433 1613 107.31 6.6% Sample 3 6088 5842 5824 6200 5988 160.58 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 561 555 488 512 529 30.29 5.7% Sample 1 561 555 488 512 529 30.29 5.7% Sample 2 1709 1635 1676 1433 1613 107.31 6.6% Sample 2 1709 1635 1676 1433 1613 107.31 6.6% Sample 3 6088 5842 5824 6200 5988 160.58 2.6% Sample 3 6088 5842 5824 6200 5988 160.58 2.6%

Expressed in the interterritorial matrix of articular deep zone cartilage (at protein level) . Isoform 1 and isoform 2 are expressed in brain. Isoform 1 is detected in lung and chondrocytes. Detected in cartilage, bone, cultured chondrocytes and lung, and at low levels in heart. Not detected in osteoblasts.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 561 1709 6088 583 2016 7238 Standard deviation 23.56 73.49 249.61 38.48 145.15 550.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 561 1709 6088 583 2016 7238 Standard deviation 23.56 73.49 249.61 38.48 145.15 550.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 561 1709 6088 583 2016 7238 Mean (pg/ml) 561 1709 6088 583 2016 7238 Standard deviation 23.56 73.49 249.61 38.48 145.15 550.09 Standard deviation 23.56 73.49 249.61 38.48 145.15 550.09 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.159 0.249 0.396 0.671 0.969 1.418 1.871 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.159 0.249 0.396 0.671 0.969 1.418 1.871 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.159 0.249 0.396 0.671 0.969 1.418 1.871 O.D. 0.046 0.159 0.249 0.396 0.671 0.969 1.418 1.871

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.