Human PTH1R ELISA Kit PicoKine®

Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as parathyroid hormone 1 receptor (PTH1R), is a protein that in humans is encoded by the PTH1R gene. It is mapped to 3p21.31. The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH) . The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 52 51 44 48 3.34 6.9% Sample 2 139 126 126 125 129 5.78 4.4% Sample 3 566 498 515 513 523 25.68 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 52 51 44 48 3.34 6.9% Sample 1 46 52 51 44 48 3.34 6.9% Sample 2 139 126 126 125 129 5.78 4.4% Sample 2 139 126 126 125 129 5.78 4.4% Sample 3 566 498 515 513 523 25.68 4.9% Sample 3 566 498 515 513 523 25.68 4.9%

Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor; PTH/PTHr receptor; Parathyroid hormone 1 receptor; PTH1 receptor; PTH1R; PTHR; PTHR1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 139 566 52 171 643 Standard deviation 2.21 7.23 30.56 2.65 9.41 40.51 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 139 566 52 171 643 Standard deviation 2.21 7.23 30.56 2.65 9.41 40.51 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 139 566 52 171 643 Mean (pg/ml) 46 139 566 52 171 643 Standard deviation 2.21 7.23 30.56 2.65 9.41 40.51 Standard deviation 2.21 7.23 30.56 2.65 9.41 40.51 CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.3% CV (%) 4.8% 5.2% 5.4% 5.1% 5.5% 6.3%

Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.084 0.138 0.224 0.4454 0.785 1.406 2.631 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.084 0.138 0.224 0.4454 0.785 1.406 2.631 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.084 0.138 0.224 0.4454 0.785 1.406 2.631 O.D. 0.028 0.084 0.138 0.224 0.4454 0.785 1.406 2.631

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.