Rat Chordin ELISA Kit PicoKine®

Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 112 103 111 105 6.86 6.5% Sample 2 293 296 263 269 280 14.44 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 112 103 111 105 6.86 6.5% Sample 1 95 112 103 111 105 6.86 6.5% Sample 2 293 296 263 269 280 14.44 5.1% Sample 2 293 296 263 269 280 14.44 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1%

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.135 0.241 0.4624 0.802 1.423 1.848 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.135 0.241 0.4624 0.802 1.423 1.848 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.083 0.135 0.241 0.4624 0.802 1.423 1.848 O.D. 0.045 0.083 0.135 0.241 0.4624 0.802 1.423 1.848

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.