Human GIF ELISA Kit PicoKine®

Intrinsic factor (IF), also known as gastric intrinsic factor (GIF), is a glycoprotein produced by the parietal cells of the stomach. It is necessary for the absorption of vitamin B12 later on in the ileum of the small intestine. In humans, the gastric intrinsic factor protein is encoded by the GIF gene. It is mapped to 11q12.1. Haptocorrin (also known as HC, R protein, and transcobalamin I, TCN1) is another glycoprotein secreted by the salivary glands which binds to vitamin B12. Vitamin B12 is acid sensitive and in binding to transcobalamin I it can safely pass through the acidic stomach to the duodenum. In the less acidic environment of the small intestine, pancreatic enzymes digest the glycoprotein carrier and vitamin B12 can then bind to intrinsic factor

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 16 16 16 16 0.86 5.3% Sample 2 102 92 104 109 101 6.17 6.1% Sample 3 360 348 301 358 341 23.96 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 16 16 16 16 0.86 5.3% Sample 1 18 16 16 16 16 0.86 5.3% Sample 2 102 92 104 109 101 6.17 6.1% Sample 2 102 92 104 109 101 6.17 6.1% Sample 3 360 348 301 358 341 23.96 7.0% Sample 3 360 348 301 358 341 23.96 7.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 102 360 21 112 425 Standard deviation 0.72 4.39 16.56 1.07 6.27 25.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 102 360 21 112 425 Standard deviation 0.72 4.39 16.56 1.07 6.27 25.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 102 360 21 112 425 Mean (pg/ml) 18 102 360 21 112 425 Standard deviation 0.72 4.39 16.56 1.07 6.27 25.08 Standard deviation 0.72 4.39 16.56 1.07 6.27 25.08 CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9% CV (%) 4.0% 4.3% 4.6% 5.1% 5.6% 5.9%

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.074 0.121 0.23 0.369 0.6256 1.058 1.702 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.074 0.121 0.23 0.369 0.6256 1.058 1.702 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.074 0.121 0.23 0.369 0.6256 1.058 1.702 O.D. 0.032 0.074 0.121 0.23 0.369 0.6256 1.058 1.702

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.