Human Renalase ELISA Kit PicoKine®

Renalase, FAD-dependent amine oxidase is an enzyme that in humans is encoded by the RNLS gene. It is mapped to 10q23.31. Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney. The renalase protein consists of a putative secretory signal peptide (SignalP score of 0.4), a flavin adenine dinucleotide (FAD) -binding region, and an oxidase domain. At least four alternative splicing isoforms have been identified in humans (hRenalase1 to hRenalase4) . Only hRenalase1 is detected in human blood samples, which means that hRenalase2 to 4 probably have different functions than hRenalase1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 171 175 177 176 3.60 2.0% Sample 2 1100 1074 1106 1218 1124 55.30 4.9% Sample 3 3799 3638 3667 3825 3732 80.93 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 171 175 177 176 3.60 2.0% Sample 1 181 171 175 177 176 3.60 2.0% Sample 2 1100 1074 1106 1218 1124 55.30 4.9% Sample 2 1100 1074 1106 1218 1124 55.30 4.9% Sample 3 3799 3638 3667 3825 3732 80.93 2.1% Sample 3 3799 3638 3667 3825 3732 80.93 2.1%

Secreted into the blood by the kidney. Highly expressed in the kidney, expressed at lower level in heart, skeletal muscle and small intestine. Its plasma concentration is markedly reduced in patients with end-stage renal disease, as compared with healthy subjects.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 1100 3799 201 1115 3899 Standard deviation 7.42 47.30 174.75 11.66 76.94 280.73 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 1100 3799 201 1115 3899 Standard deviation 7.42 47.30 174.75 11.66 76.94 280.73 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 181 1100 3799 201 1115 3899 Mean (pg/ml) 181 1100 3799 201 1115 3899 Standard deviation 7.42 47.30 174.75 11.66 76.94 280.73 Standard deviation 7.42 47.30 174.75 11.66 76.94 280.73 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD (P) and 1,6-dihydro-beta-NAD (P) to form beta-NAD (P) +. The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.137 0.257 0.497 0.892 1.562 2.196 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.137 0.257 0.497 0.892 1.562 2.196 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.076 0.137 0.257 0.497 0.892 1.562 2.196 O.D. 0.011 0.076 0.137 0.257 0.497 0.892 1.562 2.196

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.