Mouse CREG1 ELISA Kit PicoKine®

Protein CREG1 (Cellular Repressor of E1A-stimulated Genes 1) is a protein that in humans is encoded by the CREG1 gene. It is mapped to 1; 1 H2.3. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 223 221 252 239 17.86 7.4% Sample 2 677 673 789 683 705 48.33 6.8% Sample 3 4031 3933 3479 3497 3735 249.49 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 223 221 252 239 17.86 7.4% Sample 1 262 223 221 252 239 17.86 7.4% Sample 2 677 673 789 683 705 48.33 6.8% Sample 2 677 673 789 683 705 48.33 6.8% Sample 3 4031 3933 3479 3497 3735 249.49 6.6% Sample 3 4031 3933 3479 3497 3735 249.49 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 677 4031 271 693 4247 Standard deviation 13.62 35.88 221.71 16.53 44.35 284.55 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 677 4031 271 693 4247 Standard deviation 13.62 35.88 221.71 16.53 44.35 284.55 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 262 677 4031 271 693 4247 Mean (pg/ml) 262 677 4031 271 693 4247 Standard deviation 13.62 35.88 221.71 16.53 44.35 284.55 Standard deviation 13.62 35.88 221.71 16.53 44.35 284.55 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.