Mouse Podocalyxin ELISA Kit PicoKine®

Podocalyxin-like protein 1 is a protein that in humans is encoded by the PODXL gene. It is mapped to 6 A3.3; 6 12.57 Cm. This gene encodes a member of the CD34 sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Inactivation of the encoding gene in mice leads to anuria, omphalocele and perinatal death. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 200 221 226 219 12.04 5.4% Sample 2 1061 1004 971 1073 1027 41.64 4.0% Sample 3 3061 3253 3042 3105 3115 82.74 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 232 200 221 226 219 12.04 5.4% Sample 1 232 200 221 226 219 12.04 5.4% Sample 2 1061 1004 971 1073 1027 41.64 4.0% Sample 2 1061 1004 971 1073 1027 41.64 4.0% Sample 3 3061 3253 3042 3105 3115 82.74 2.6% Sample 3 3061 3253 3042 3105 3115 82.74 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 1061 3061 281 1101 3658 Standard deviation 13.69 66.84 186.72 18.55 74.87 263.38 CV (%) 5.9% 6.3% 6.1% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 232 1061 3061 281 1101 3658 Standard deviation 13.69 66.84 186.72 18.55 74.87 263.38 CV (%) 5.9% 6.3% 6.1% 6.6% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 232 1061 3061 281 1101 3658 Mean (pg/ml) 232 1061 3061 281 1101 3658 Standard deviation 13.69 66.84 186.72 18.55 74.87 263.38 Standard deviation 13.69 66.84 186.72 18.55 74.87 263.38 CV (%) 5.9% 6.3% 6.1% 6.6% 6.8% 7.2% CV (%) 5.9% 6.3% 6.1% 6.6% 6.8% 7.2%

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.082 0.135 0.214 0.402 0.731 1.288 2.162 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.082 0.135 0.214 0.402 0.731 1.288 2.162 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.082 0.135 0.214 0.402 0.731 1.288 2.162 O.D. 0.033 0.082 0.135 0.214 0.402 0.731 1.288 2.162

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.