Human AKR1C4 ELISA Kit PicoKine®

3α-Hydroxysteroid dehydrogenase (3α-HSD or aldo-keto reductase family 1 member C4) is an enzyme that in humans is encoded by the AKR1C4 gene. It is mapped to 10p15.1. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 227 184 180 201 15.72 7.8% Sample 2 913 1148 825 825 928 60.12 6.5% Sample 3 4596 3381 3941 4167 4021 141.55 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 227 184 180 201 15.72 7.8% Sample 1 211 227 184 180 201 15.72 7.8% Sample 2 913 1148 825 825 928 60.12 6.5% Sample 2 913 1148 825 825 928 60.12 6.5% Sample 3 4596 3381 3941 4167 4021 141.55 3.5% Sample 3 4596 3381 3941 4167 4021 141.55 3.5%

Aldo-keto reductase family 1 member C4; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4; CHDR

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 913 4596 295 1047 3034 Standard deviation 12.74 30.49 165.92 15.93 44.18 207.22 CV (%) 6.0% 3.3% 3.6% 5.4% 4.2% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 913 4596 295 1047 3034 Standard deviation 12.74 30.49 165.92 15.93 44.18 207.22 CV (%) 6.0% 3.3% 3.6% 5.4% 4.2% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 211 913 4596 295 1047 3034 Mean (pg/ml) 211 913 4596 295 1047 3034 Standard deviation 12.74 30.49 165.92 15.93 44.18 207.22 Standard deviation 12.74 30.49 165.92 15.93 44.18 207.22 CV (%) 6.0% 3.3% 3.6% 5.4% 4.2% 6.8% CV (%) 6.0% 3.3% 3.6% 5.4% 4.2% 6.8%

Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) . Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.115 0.185 0.337 0.62 1.078 1.62 2.09 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.115 0.185 0.337 0.62 1.078 1.62 2.09 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.115 0.185 0.337 0.62 1.078 1.62 2.09 O.D. 0.051 0.115 0.185 0.337 0.62 1.078 1.62 2.09

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.