Human SLITRK6 ELISA Kit PicoKine®

SLIT and NTRK-like protein 6 is a protein that in humans is encoded by the SLITRK6 gene. It is mapped to 13q31.1. This gene encodes a member of the SLITRK protein family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. This protein functions as a regulator of neurite outgrowth required for normal hearing and vision. Mutations in this gene are a cause of myopia and deafness.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 27 27 25 25 2.04 8.1% Sample 2 71 63 66 74 68 4.27 6.2% Sample 3 307 320 287 314 307 12.42 4.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 27 27 25 25 2.04 8.1% Sample 1 22 27 27 25 25 2.04 8.1% Sample 2 71 63 66 74 68 4.27 6.2% Sample 2 71 63 66 74 68 4.27 6.2% Sample 3 307 320 287 314 307 12.42 4.0% Sample 3 307 320 287 314 307 12.42 4.0%

In adult brain, highly expressed in putamen with no expression in cerebral cortex. Expressed in adult and fetal lung and fetal liver. Also expressed at high levels in some brain tumors including medulloblastomas and primitive neuroectodermal tumors.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 71 307 28 84 421 Standard deviation 1.08 3.76 16.58 1.85 5.71 33.26 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 71 307 28 84 421 Standard deviation 1.08 3.76 16.58 1.85 5.71 33.26 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 71 307 28 84 421 Mean (pg/ml) 22 71 307 28 84 421 Standard deviation 1.08 3.76 16.58 1.85 5.71 33.26 Standard deviation 1.08 3.76 16.58 1.85 5.71 33.26 CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9% CV (%) 4.9% 5.3% 5.4% 6.6% 6.8% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.101 0.178 0.312 0.633 0.982 1.525 2.047 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.101 0.178 0.312 0.633 0.982 1.525 2.047 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.101 0.178 0.312 0.633 0.982 1.525 2.047 O.D. 0.01 0.101 0.178 0.312 0.633 0.982 1.525 2.047

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.