Human SLITRK1 ELISA Kit PicoKine®

SLIT and NTRK-like protein 1 is a protein that in humans is encoded by the SLITRK1 gene. It is mapped to 13q31.1. This gene encodes a member of the SLITRK protein family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. However, the protein encoded by this gene lacks the region of homology to neurotrophin receptors. This protein is thought to be involved in neurite outgrowth. Mutations in this gene may be associated with Tourette syndrome. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 17 19 17 17 1.08 6.3% Sample 2 90 88 100 99 94 5.30 5.6% Sample 3 300 274 280 317 292 16.99 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 17 19 17 17 1.08 6.3% Sample 1 16 17 19 17 17 1.08 6.3% Sample 2 90 88 100 99 94 5.30 5.6% Sample 2 90 88 100 99 94 5.30 5.6% Sample 3 300 274 280 317 292 16.99 5.8% Sample 3 300 274 280 317 292 16.99 5.8%

Expressed predominantly in the frontal lobe of the cerebral cortex of the brain. Also expressed in some astrocytic brain tumors such as astrocytomas, oligodendrogliomas, glioblastomas, gangliogliomas and primitive neuroectodermal tumors.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 90 300 25 113 441 Standard deviation 0.82 4.77 16.50 1.45 7.01 29.11 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 90 300 25 113 441 Standard deviation 0.82 4.77 16.50 1.45 7.01 29.11 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 90 300 25 113 441 Mean (pg/ml) 16 90 300 25 113 441 Standard deviation 0.82 4.77 16.50 1.45 7.01 29.11 Standard deviation 0.82 4.77 16.50 1.45 7.01 29.11 CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6% CV (%) 5.1% 5.3% 5.5% 5.8% 6.2% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.171 0.273 0.435 0.687 1.143 1.813 2.707 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.171 0.273 0.435 0.687 1.143 1.813 2.707 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.171 0.273 0.435 0.687 1.143 1.813 2.707 O.D. 0.017 0.171 0.273 0.435 0.687 1.143 1.813 2.707

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.