Mouse CD300b ELISA Kit PicoKine®

CD300 molecule like family member b is a protein that in humans is encoded by the CD300LB gene. It is mapped to 11; 11 E2. CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 21 19 20 1.00 5.0% Sample 2 70 67 57 66 65 4.84 7.4% Sample 3 278 270 278 255 270 9.39 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 21 19 20 1.00 5.0% Sample 1 19 21 21 19 20 1.00 5.0% Sample 2 70 67 57 66 65 4.84 7.4% Sample 2 70 67 57 66 65 4.84 7.4% Sample 3 278 270 278 255 270 9.39 3.4% Sample 3 278 270 278 255 270 9.39 3.4%

CMRF35-like molecule 7; CLM-7; CD300 antigen-like family member B; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; CD300b; Cd300lb; Irem3; Lmir5

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.