Mouse CD300b ELISA Kit PicoKine®

Mouse CD300b ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cd300lb in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD300 molecule like family member b is a protein that in humans is encoded by the CD300LB gene. It is mapped to 11; 11 E2. CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 21 19 20 1.00 5.0% Sample 2 70 67 57 66 65 4.84 7.4% Sample 3 278 270 278 255 270 9.39 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 21 21 19 20 1.00 5.0% Sample 1 19 21 21 19 20 1.00 5.0% Sample 2 70 67 57 66 65 4.84 7.4% Sample 2 70 67 57 66 65 4.84 7.4% Sample 3 278 270 278 255 270 9.39 3.4% Sample 3 278 270 278 255 270 9.39 3.4%

Synonyms

CMRF35-like molecule 7; CLM-7; CD300 antigen-like family member B; Immune receptor expressed on myeloid cells 3; IREM-3; Leukocyte mono-Ig-like receptor 5; CD300b; Cd300lb; Irem3; Lmir5

Gene Name

Cd300lb

Gene ID

217304

UniProt

Q3U497

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I18-P209

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in myeloid cells.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 70 278 23 118 418 Mean (pg/ml) 19 70 278 23 118 418 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 Standard deviation 0.78 3.29 13.62 1.17 6.49 26.33 CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3% CV (%) 4.1% 4.7% 4.9% 5.1% 5.5% 6.3%

Sensitivity

<10 pg/mL

Function

Acts as an activating immune receptor in mast cells through its interaction with ITAM-bearing adapter TYROBP.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CD300b

Anticoagulant

Heparin or EDTA

Gene Name Synonym

CD300 molecule like family member B

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287 O.D. 0.033 0.15 0.219 0.393 0.725 1.241 1.902 2.287

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.