Mouse SEZ6L ELISA Kit PicoKine®

Mouse SEZ6L ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Sez6l in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. It is mapped to 5; 5 F.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 119 109 109 125 115 6.83 5.9% Sample 2 516 625 523 552 554 43.15 7.7% Sample 3 2469 2400 2352 2325 2386 54.68 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 119 109 109 125 115 6.83 5.9% Sample 1 119 109 109 125 115 6.83 5.9% Sample 2 516 625 523 552 554 43.15 7.7% Sample 2 516 625 523 552 554 43.15 7.7% Sample 3 2469 2400 2352 2325 2386 54.68 2.2% Sample 3 2469 2400 2352 2325 2386 54.68 2.2%

Synonyms

Seizure 6-like protein; Acupuncture-induced protein 1-L; Brain-specific receptor-like protein B; BSRP-B; Sez6l; Aig1l; Kiaa0927

Gene Name

Sez6l

Gene ID

56747

UniProt

Q6P1D5

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-A958

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed exclusively in the brain, predomimantly in neurons. Wide expression in the gray matter of the brain with high levels in the olfactory bulb, anterior olfactory nuclei, hippocampal formation and cerebellar cortex. Detected diffusely and weakly in the white matter, such as the corpus callosum and cerebellar medulla. In the cerebellar cortex, intensely expressed in Purkinje cells and granule cells. Detected also in interneurons in the molecular layer.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 119 516 2469 129 562 2481 Standard deviation 5.71 27.35 138.26 6.58 32.03 153.82 CV (%) 4.8% 5.3% 5.6% 5.1% 5.7% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 119 516 2469 129 562 2481 Standard deviation 5.71 27.35 138.26 6.58 32.03 153.82 CV (%) 4.8% 5.3% 5.6% 5.1% 5.7% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 119 516 2469 129 562 2481 Mean (pg/ml) 119 516 2469 129 562 2481 Standard deviation 5.71 27.35 138.26 6.58 32.03 153.82 Standard deviation 5.71 27.35 138.26 6.58 32.03 153.82 CV (%) 4.8% 5.3% 5.6% 5.1% 5.7% 6.2% CV (%) 4.8% 5.3% 5.6% 5.1% 5.7% 6.2%

Sensitivity

<50 pg/mL

Function

Candidate tumor suppressor gene. May contribute to specialized endoplasmic reticulum functions in neurons.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SEZ6L

Anticoagulant

Heparin

Gene Name Synonym

Seizure related 6 homolog like

Subcellular Location

Cell membrane. Single-pass type I membrane protein. Endoplasmic reticulum membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.05 0.116 0.17 0.267 0.535 0.876 1.383 1.964 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.05 0.116 0.17 0.267 0.535 0.876 1.383 1.964 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.05 0.116 0.17 0.267 0.535 0.876 1.383 1.964 O.D. 0.05 0.116 0.17 0.267 0.535 0.876 1.383 1.964

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.