Human EDAR ELISA Kit PicoKine®

Human EDAR ELISA Kit PicoKine® (96 Tests) . Quantitate Human EDAR in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ectodysplasin A receptor (EDAR) is a protein that in humans is encoded by the EDAR gene. It is mapped to 2q13. This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 20 21 22 21 0.70 3.3% Sample 2 75 91 75 75 79 6.92 8.7% Sample 3 384 433 444 406 416 23.42 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 20 21 22 21 0.70 3.3% Sample 1 21 20 21 22 21 0.70 3.3% Sample 2 75 91 75 75 79 6.92 8.7% Sample 2 75 91 75 75 79 6.92 8.7% Sample 3 384 433 444 406 416 23.42 5.6% Sample 3 384 433 444 406 416 23.42 5.6%

Synonyms

Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless homolog; EDA-A1 receptor; Ectodermal dysplasia receptor; Ectodysplasin-A receptor; EDAR; DL

Gene Name

EDAR

Gene ID

10913

UniProt

Q9UNE0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E27-A187

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Detected in fetal kidney, lung, skin and cultured neonatal epidermal keratinocytes. Not detected in lymphoblast and fibroblast cell lines.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human EDAR

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Ectodysplasin A receptor

Subcellular Location

Membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.