Human Omentin/intelectin-1 ELISA Kit PicoKine®

Intelectin-1, also known as omentin or intestinal lactoferrin receptor, is an intelectin encoded in humans by the ITLN1 gene. It is mapped to 1q23.3. Intelectin-1 functions both as a receptor for bacterial arabinogalactans and for lactoferrin. Having conserved ligand binding site residues, both human and mouse intelectin-1 bind the exocyclic vicinal diol of carbohydrate ligands such as galactofuranose.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 112 103 111 105 6.86 6.5% Sample 2 293 296 263 269 280 14.44 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 112 103 111 105 6.86 6.5% Sample 1 95 112 103 111 105 6.86 6.5% Sample 2 293 296 263 269 280 14.44 5.1% Sample 2 293 296 263 269 280 14.44 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1% Sample 3 1596 1398 1585 1542 1530 78.97 5.1%

Intelectin-1; ITLN-1; Endothelial lectin HL-1; Galactofuranose-binding lectin; Intestinal lactoferrin receptor; Omentin; ITLN1; INTL; ITLN; LFR; UNQ640; PRO1270

Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level) (PubMed:16531507) . Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1% CV (%) 5.1% 5.3% 5.6% 6.6% 6.8% 7.1%

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner. Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) . Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind human glycans. Probably plays a role in the defense system against microorganisms. May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.141 0.204 0.354 0.63 1.057 1.942 2.457 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.141 0.204 0.354 0.63 1.057 1.942 2.457 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.068 0.141 0.204 0.354 0.63 1.057 1.942 2.457 O.D. 0.068 0.141 0.204 0.354 0.63 1.057 1.942 2.457

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.